Cryo Mix is a powerful AI-based music mixing and mastering tool that includes an AI Audio Separator. It can break down stems like vocals, drums and instruments from a single file using machine learning technology. It's got an interface that's easy to use, too, and other features like pitch correction and final mix preparation.
Another tool worth a look is AudioShake, which is geared specifically for audio separation and isolation. It can recognize and isolate audio elements from files up to 192kHz, so it's good for a variety of tasks, including making instrumentals and analyzing audio.
If you want something a bit more streamlined, LALAL.AI offers a fast and accurate way to split an audio file into vocal and instrumental tracks. You can upload multiple files and choose from a variety of stem options for fast processing and good results. The service is geared for musicians, streamers and transcribers.